Alberobello and Locorotondo stand out as prime destinations for wine lovers exploring Puglia. The region’s unique landscape of trulli houses, dry stone walls, and rolling vineyards creates a perfect backdrop for memorable wine experiences. Some tours, like the E-Bike, Focaccia and Wine, combine active exploration with local tastings, allowing you to pedal through scenic countryside while savoring authentic Apulian flavors. Others, like the Wine and Food Pairing at a local winery, offer intimate, guided tastings paired with regional dishes, perfect for food and wine enthusiasts. For those who prefer a self-guided adventure, a GPS-based bike tour delivers flexibility and a dash of adventure along historic aqueduct routes. Whichever you choose, each experience showcases the region’s famous wines and scenic charm, making them excellent options for a day out.
Below, you’ll find a curated list of the top six wine tours in Alberobello and Locorotondo, each offering a different way to experience Puglia’s viticulture and culture — from active e-biking trips to relaxed wine tastings.
1. E-Bike, Focaccia and Wine

At number one, the E-Bike, Focaccia and Wine tour offers a lively, engaging way to explore the Valle d’Itria countryside. This 3-hour adventure is perfect for those who want to combine physical activity with authentic food and drink. Starting from Alberobello, the “trulli capital,” you’ll pedal 20 km (about 12 miles) through lush fields, ancient farmhouses, and dry-stone walls, soaking in the rural landscape that defines this part of Italy. Your guide shares personal favorites and local stories, making it more than just a sightseeing ride.
The highlight is a stop at Locorotondo, considered one of Italy’s most charming villages, where you’ll visit Giovanni’s bakery for freshly baked Apulian focaccia. This focaccia, filled with your choice of charcuterie and vegetables, is paired with a glass of regional wine—something you’ll savor while chatting with locals. The tour finishes back in Alberobello, with a taste of local almond cookies. Rated 5.0/5 from 32 reviews, this trip offers a friendly, relaxed vibe and a chance to see the countryside through a local’s eyes. It’s ideal for active travelers who enjoy genuine local interactions and tasty bites.
Bottom Line: A balanced mix of cycling, food, and wine, this tour is perfect for those who want a laid-back, authentic experience with a dash of adventure.
2. Ebike Tours: Alberobello and the Wines of Valle d’Itria

Coming in at number two, the Ebike Tours: Alberobello and the Wines of Valle d’Itria offers a 4-hour journey that combines scenic cycling with visits to historic wineries. This small-group tour starts from Martina Franca and takes you through the quintessential vineyards and olive groves of the region, ending in Alberobello’s famous trulli district. Along the way, you’ll enjoy the freedom of pedaling on rural country roads without the hassle of navigation, thanks to your guide.
The tour’s highlight is a visit to an 18th-century wine estate nestled in the Murgia plain. Here, you get a guided tour of the vineyards and production facilities, followed by a wine tasting of local reds and whites. This experience is highly rated, with reviews praising the knowledgeable guides and the quality of wines. At €85.98 per person, it offers excellent value considering the combination of active exploration and culture.
Bottom Line: Ideal for active travelers who want to explore the countryside and enjoy a genuine wine-tasting experience at a historic estate.
3. GPS Tour with E-Bike on Cycleroute of Acqueduct Pugliese with Wine Tasting

For those seeking independence mixed with guided insights, the GPS tour with E-Bike on the cycleroute of Acqueduct Pugliese ranks third. This self-guided ride allows you to follow preloaded tracks along the historic aqueduct and surrounding countryside. Over 3 to 4 hours, you’ll visit scenic spots like the Valle d’Itria, Locorotondo’s charming center, and finish with a wine tasting right in a vineyard.
Rated 4.5/5, this tour appeals to couples, families, or groups of friends who prefer the flexibility of riding at their own pace. The bikes are high-quality, and the GPS provides clear guidance, making navigation straightforward. The final wine tasting is a great way to cap off the day, with a chance to savor regional whites in the historic town of Locorotondo.
Bottom Line: It’s a flexible, independent option for confident cyclists eager for a scenic ride with a memorable wine tasting at the end.
4. Wine and Food Pairing Experience at Alberobello Winery

Positioned at number four, the Wine and Food Pairing Experience focuses on the culinary side of wine appreciation. This 2.5-hour session is ideal for foodies and romantics alike. Led by a sommelier, you’ll sample four local wines— including Cabernet Sauvignon—each paired with regional dishes crafted from organic ingredients.
The winery’s setting offers stunning vineyard views, and the guide shares stories about local wine-making traditions. The tour has received high praise, with reviews noting the “delicious wine and food pairings” and the insightful guidance from knowledgeable staff. The experience offers excellent value at $63.62 per person, especially for those who want to combine learning with tasting in a relaxed setting.
Bottom Line: A perfect choice for wine lovers who also enjoy discovering regional cuisine in a cozy, authentic environment.
5. Wine Tasting

At number five, the Wine Tasting session provides a straightforward way to sample the region’s signature wines. Spanning about 2 hours and costing $31.81, this private experience covers a range of reds and whites from Itria Valley and Salento, including Manduria Primitivo and Negroamaro. You’ll learn about each wine’s terroir and production process while tasting regional specialties like taralli and bruschette.
Guests find this tasting to be intimate and educational. Reviewers mention the “excellent service” and the chance to get a well-rounded introduction to Puglia’s wine heritage without a lengthy commitment. It’s ideal if you want a quick, quality sampling that complements other sightseeing.
Bottom Line: An affordable, educational introduction to regional wines, suited for those with limited time or budget.
6. Discover Valle d’Itria and Its Wines by E-Bike

Completing the list, the Discover Valle d’Itria and Its Wines by E-Bike offers a 4-hour ride through the most remote corners of the valley. Starting from Martina Franca, you’ll cover around 30 km on easy tracks along sheep trails and rural paths, reaching the famous aqueduct route. This tour is perfect for those wanting to explore lesser-seen parts of the region while enjoying stops at a vineyard for a tasting.
Rated 4.0/5 from one reviewer, it’s a good choice for adventurous bikers who want off-the-beaten-path views and a taste of local wine. The tour provides a mixture of rural scenery and local culture, with the optional visit to one of the region’s top wineries.
Bottom Line: Best suited for active travelers eager to explore the hidden parts of Valle d’Itria while sampling regional wines.
How to Choose the Right Wine Tour in Alberobello and Locorotondo
When selecting a wine tour, consider your budget, fitness level, and interests. If you love active outings, the E-Bike, Focaccia and Wine or Discover Valle d’Itria by e-bike offer scenic cycling with tasty rewards. For a relaxed, culinary-focused experience, the Wine and Food Pairing provides an intimate setting. Budget-wise, the Wine Tasting is an affordable way to sample regional wines without a long tour commitment.
Timing can also influence your choice — summer months bring warm weather perfect for outdoor tasting and cycling, but always book early in high season. For a comprehensive experience, combining a guided tour with tasting at a local winery can deepen your understanding of Puglia’s winemaking traditions.
Final Thoughts
Alberobello and Locorotondo offer a rich variety of wine experiences that suit every traveler. Whether you prefer pedaling through vineyards and charming villages, enjoying a leisurely tasting, or exploring remote countryside routes, there’s a perfect option for you. The E-Bike, Focaccia and Wine stands out for its lively pace and authentic feel, while the Wine and Food Pairing is ideal for those looking for a culinary adventure. For flexible, independent explorers, the GPS bike tour offers freedom with guided insights.
Remember to book early, especially in peak months, and choose an experience that matches your energy, interests, and budget. Puglia’s wines, landscapes, and warm hospitality await you—cheers to unforgettable tastings!



